Measurement

Part:BBa_K2115000:Design

Designed by: Antonella Elizabeth Osses Toledo; Javier David Villacreses Lucero   Group: iGEM16_Saint_Rose_School_A   (2016-10-14)


It detects carbon monoxide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 677
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The only consideration was the bioinformatic assembly this parts, because we had to learn to use Artemis software.


Source

LacI: genomically it comes from E.coli bacterium, however we took this promoter from the IGEM catalog. RBS: ribosome binding site. CooA: a transcription factor present in the Rhodospirillum Rubrum protobacteria. Terminators: an RNA sequence that usually occurs at the end of a gene or operon mRNA and causes transcription to stop.


References